Himanshu Sethi

Compositions And Methods For Identifying Nucleic Acid Molecules

Application

#2018015 Jun 7, 2018

Filed

Sep 26, 2017

Inventor

Ryan SWENERTON (San Bruno, CA) · Matthew RABINOWITZ (San Francisco, CA) · Styrmir SIGURJONSSON (San Jose, CA) · George GEMELOS (Portland, OR) · Apratim GANGULY (Daly City, CA) · Himanshu SETHI (Sunnyvale, CA)

Appl. No.

15/716331

Assignee

Natera, Inc. (San Carlos, CA)

International Classification

C12Q 1/68

Abstract

The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

Novel Primers And Uses Thereof

Application

#2021007 Mar 11, 2021

Filed

Jan 11, 2019

Inventor

Ryan SWENERTON (Millbrae, CA) · Scott DASHNER (Sunnyvale, CA) · Fei LU (Fremont, CA) · Himanshu SETHI (Sunnyvale, CA)

Appl. No.

16/959949

Assignee

Natera, Inc. (San Carlos, CA)

International Classification

C12Q 1/6853, C12Q 1/6858

Abstract

Disclosed here is a composition comprising a primer that is (a) a loopable primer comprising a target-specific section, an adaptor section, and a stem-forming section, wherein the stem-forming section is hybridizable to a portion of the target-specific section to form a stem structure, or (b) a split primer comprising a first target-specific section, a second target-specific section, and an adaptor section positioned between the first target-specific section and the second target-specific section, or (c) a split-loopable primer comprising a first target-specific section, a second target-specific section, a stem-forming section positioned between the first target-specific section and the second target-specific section, and an adaptor section, or comprising a first adaptor section, a second adaptor section, a stem-forming section positioned between the first adaptor section and the second adaptor section, and a target-specific section. Also disclosed is a method for amplifying a target locus of interest from a template DNA, comprising at least two pre-amplification cycles using the loopable primer, the split primer and/or the split-loopable primer, wherein each amplification cycle comprises annealing the primer to the template DNA or pre-amplification product thereof and elongating the annealed primer. Further disclosed is a kit for amplifying a target locus of interest, comprising the loopable primer, the split primer, and/or the split-loopable primer.

Compositions And Methods For Identifying Nucleic Acid Molecules

Application

#2018015 Jun 7, 2018

Filed

Sep 26, 2017

Inventor

Ryan SWENERTON (San Bruno, CA) · Matthew RABINOWITZ (San Francisco, CA) · Styrmir SIGURJONSSON (San Jose, CA) · George GEMELOS (Portland, OR) · Apratim GANGULY (Daly City, CA) · Himanshu SETHI (Sunnyvale, CA)

Appl. No.

15/716058

Assignee

Natera, Inc. (San Carlos, CA)

International Classification

C12Q 1/68

Abstract

The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

Methods For Cancer Detection And Monitoring

Application

#2019031 Oct 17, 2019

Filed

Apr 12, 2019

Inventor

Raheleh SALARI (San Carlos, CA) · Ryan SWENERTON (San Bruno, CA) · Hsin-Ta WU (Sunnyvale, CA) · Himanshu SETHI (Sunnyvale, CA)

Appl. No.

16/382520

Assignee

Natera, Inc. (San Carlos, CA)

International Classification

C12Q 1/6827, G16H 50/30, C12Q 1/6886

Abstract

The invention provides methods for detecting single nucleotide variants in breast cancer, bladder cancer, or colorectal cancer. Additional methods and compositions, such as reaction mixtures and solid supports comprising clonal populations of nucleic acids, are provided. For example, provided here is a method for monitoring and detection of early relapse or metastasis of breast cancer, bladder cancer, or colorectal cancer, comprising generating a set of amplicons by performing a multiplex amplification reaction on nucleic acids isolated from a sample of blood or urine or a fraction thereof from a patient who has been treated for a breast cancer, bladder cancer, or colorectal cancer, wherein each amplicon of the set of amplicons spans at least one single nucleotide variant locus of a set of patient-specific single nucleotide variant loci associated with the breast cancer, bladder cancer, or colorectal cancer; and determining the sequence of at least a segment of each amplicon of the set of amplicons that comprises a patient-specific single nucleotide variant locus, wherein detection of one or more patient-specific single nucleotide variants is indicative of early relapse or metastasis of breast cancer, bladder cancer, or colorectal cancer.

Compositions And Methods For Identifying Nucleic Acid Molecules

Application

#2018015 Jun 7, 2018

Filed

Dec 7, 2016

Inventor

Ryan SWENERTON (San Bruno, CA) · Matthew RABINOWITZ (San Francisco, CA) · Styrmir SIGURJONSSON (San Jose, CA) · George GEMELOS (Portland, OR) · Apratim GANGULY (Daly City, CA) · Himanshu SETHI (Sunnyvale, CA)

Appl. No.

15/372279

Assignee

Natera, Inc. (San Carlos, CA)

International Classification

C12Q 1/68

Abstract

The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

Compositions And Methods For Identifying Nucleic Acid Molecules

Application

#2019030 Oct 10, 2019

Filed

May 21, 2019

Inventor

Ryan SWENERTON (San Bruno, CA) · Matthew RABINOWITZ (San Francisco, CA) · Styrmir SIGURJONSSON (San Jose, CA) · George GEMELOS (Portland, OR) · Apratim GANGULY (Daly City, CA) · Himanshu SETHI (Sunnyvale, CA)

Appl. No.

16/418104

Assignee

Natera, Inc. (San Carlos, CA)

International Classification

C12Q 1/6869, C12Q 1/6806

Abstract

The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.