The University

Promotion Of Somatic Embryogenesis In Plants By Wuschel Gene Expression

Application

#2013020 Aug 8, 2013

Filed

Apr 4, 2013

Inventor

Qi-Wen NIU (Staten Island, NY) · Giovanna FRUGIS (Rome, IT) · Nam-Hai CHUA (New York, NY)

Appl. No.

13/856623

Assignee

The Rockefeller University (New York, NY)

International Classification

C12N 15/82

U.S. Classification

800278

Abstract

The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

Methods To Fix And Detect Nucleic Acids

Application

#2013013 May 23, 2013

Filed

Feb 5, 2013

Inventor

John Pena (New York, NY) · Pavol Cekan (New York, NY) · Cherin Sohn (Rego Park, NY) · Sara H. Rouhanifard (New York, NY) · Janos Ludwig (Bonn-Venusberg, DE)

Appl. No.

13/759363

Assignee

THE ROCKEFELLER UNIVERSITY (New York, NY)

International Classification

G01N 1/30

U.S. Classification

435 611

Abstract

In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide.

Modified Rna Ligase For Efficient 3` Modification Of Rna

Application

#2013013 May 23, 2013

Filed

Jan 25, 2013

Inventor

Janos Ludwig (New York, NY) · Yi Pei (New York, NY) · Carolina Lin (New York, NY)

Appl. No.

13/750522

Assignee

THE ROCKEFELLER UNIVERSITY (New York, NY)

International Classification

C12N 9/00

U.S. Classification

435 913

Abstract

The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3′ hydroxyl group of RNA in the absence of ATP using the ligase.

Protein And Nucleic Acid Delivery Vehicles, Components And Mechanisms Thereof

Application

#2013019 Aug 1, 2013

Filed

Mar 12, 2013

Inventor

Venigalla B. Rao (Silver Spring, MD)

Appl. No.

13/796263

Assignee

THE CATHOLIC UNIVERSITY OF AMERICA (Washington, DC)

International Classification

C12N 15/88

U.S. Classification

4352351

Abstract

Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.

Microrna And Methods For Inhibiting Same

Application

#2013013 May 23, 2013

Filed

Jan 18, 2013

Inventor

Matthew N. Poy (New York, NY) · Thomas H. Tuschl (New York, NY)

Appl. No.

13/745163

Assignee

THE ROCKEFELLER UNIVERSITY (New York, NY)

International Classification

C07H 21/02

U.S. Classification

4353201, 536 231, 530322

Abstract

The invention relates to isolated DNA or RNA molecules comprising at least ten contiguous bases having a sequence in a pancreatic islet microRNA. In another embodiment, the invention relates to isolated single stranded pancreatic islet microRNA molecules or anti-pancreatic islet microRNA molecules.